郭一凡,张京伟,范晓洁,王文娟,封棣.中国市售食品接触硅胶制品的细胞毒性评价[J].包装工程,2023,44(19):42-49.
GUO Yi-fan,ZHANG Jing-wei,FAN Xiao-jie,WANG Wen-juan,FENG Di.Cytotoxicity Evaluation of Food Contact Silicone Products Marketed in China[J].Packaging Engineering,2023,44(19):42-49. |
| 中国市售食品接触硅胶制品的细胞毒性评价 |
| Cytotoxicity Evaluation of Food Contact Silicone Products Marketed in China |
| 投稿时间：2023-04-15 |
| DOI：10.19554/j.cnki.1001-3563.2023.19.006 |
| 中文关键词: 食品接触硅胶制品 模拟迁移 细胞毒性 |
| 英文关键词: food contact silicone products simulated migration cytotoxicity |
| 基金项目:国家自然科学基金面上项目(31871722) |
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| 中文摘要: |
| 目的 为了研究不同细胞毒性测试方法之间的差别，本研究利用不同体外细胞毒性评价测试体系下的食品接触硅胶制品(Food Contact Silicone Products, FCSPs)的整体安全性进行研究。方法 样品在体积分数为95%的乙醇中模拟迁移(70℃保持2 h)后，通过系统优化实验条件，发展了FCSPs迁移液的细胞毒性评价方法。利用HeLa(子宫颈癌细胞)-NRU、HeLa-CCK-8(CellCountingKit-8，CCK-8)、HepG2(Human Hepatoblastoma)-NRU、HepG2-CCK-8共4种测试体系对中国市售的19种FCSPs进行了细胞毒性评价。结果 样品在不同测试体系下的细胞毒性结果不同，其中65%的样品呈现中度毒性。结论 FCSPs的整体生物安全性亟须关注并深入研究。本研究为食品接触材料的体外生物测定提供了研究思路和科学依据。 |
| 英文摘要: |
| The work aims to study the overall safety of food contact silicone products (FCSPs) by different in vitro cytotoxicity evaluation and test systems to study the difference of different in vitro cytotoxicity test methods. After the samples were simulated in 95% ethanol at 70 ℃ for 2 h, the cytotoxicity evaluation method of FCSPs migration was developed by systematically optimizing the experimental conditions. Four test systems including HeLa-NRU, HeLa-CCK-8, HepG2-NRU and HepG2-CCK-8 were used to evaluate the cytotoxicity of 19 FCSPs marketed in China. The samples showed different cytotoxicity results under different testing systems. Among them, 65% of the samples showed moderate cytotoxicity. The overall biological safety of FCSPs needs urgent attention and in-depth study. This study provides research ideas and scientific basis for the in vitro bioassay of food contact materials. |
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