张家涛,武娇,杨华,王红雨,徐赵萌,孙彤.原位合成纳米SiOx/LZM/TP-CS涂膜对腐败希瓦氏菌的抑菌机理[J].包装工程,2021,42(23):76-83.
ZHANG Jia-tao,WU Jiao,YANG Hua,WANG Hong-yu,XU Zhao-meng,SUN Tong.Antibacterial Mechanism of the In-Situ Synthesis of Nano-SiOx/LZM/TP-CS Composite Coatings against Shewanella Putrefaciens[J].Packaging Engineering,2021,42(23):76-83. |
| 原位合成纳米SiOx/LZM/TP-CS涂膜对腐败希瓦氏菌的抑菌机理 |
| Antibacterial Mechanism of the In-Situ Synthesis of Nano-SiOx/LZM/TP-CS Composite Coatings against Shewanella Putrefaciens |
| 投稿时间：2021-04-14 |
| DOI：10.19554/j.cnki.1001-3563.2021.23.011 |
| 中文关键词: 复合保鲜涂膜 腐败希瓦氏菌 抑菌机理 |
| 英文关键词: composite preservative coatings Shewanella putrefaciens antibacterial mechanism |
| 基金项目:辽宁省一流学科专项资金(LSPBD20304，LSPBD20310) |
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| 中文摘要: |
| 目的 为拓展壳聚糖(CS)保鲜涂膜在水产品中的应用，并为水产品保鲜机制的研究提供理论支持。方法 以原位合成纳米SiOx改性的CS为成膜基质，以溶菌酶(LZM)和茶多酚(TP)为添加剂，采用流延法制备复合保鲜涂膜。以水产品中优势腐败菌——腐败希瓦氏菌为抑菌对象，研究涂膜的抑菌性能，用扫描电子显微镜(SEM)观测复合保鲜涂膜处理前后菌体的微观形貌变化，测定复合保鲜涂膜处理一定时间后菌悬液的电导率、OD260值、Na+/K+-ATP酶和AKP酶活性的变化。结果 各涂膜均表现出了抑菌性能，涂膜处理后菌体变形，且其菌悬液的电导率、OD260值、Na+/K+-ATP酶和AKP酶活性明显高于同期未处理样品。CS经纳米SiOx改性后，涂膜的抑菌性能略有提高，菌体多处塌陷，菌悬液电导率、OD260值、Na+/K+-ATP酶和AKP酶活性显著增加;继续加入LZM或TP后，涂膜的抑菌性能继续提高，且原位合成纳米SiOx/TP-CS涂膜抑菌性能更优。LZM和TP同时加入经纳米SiOx改性的CS涂膜中后，复合涂膜处理的菌体细胞壁溶解，损伤严重，同期处理的菌悬液电导率、OD260值最高，并对Na+/K+-ATP酶、AKP酶活性有抑制作用。结论 结合前期的研究结果，SiOx/LZM/TP-CS复合保鲜涂膜具有良好的保鲜、抑菌性能，可为可食性保鲜涂膜的应用提供理论指导和技术支持。 |
| 英文摘要: |
| The work aims to expand the use of chitosan (CS) in preservative coatings and provide theoretical support for research of aquatic product preservation mechanism. With chitosan (CS) modified by in-situ synthesis of nano-SiOx as the film-forming matrix, and lysozyme (LZM) and tea polyphenols (TP) as additives, the in-situ synthesis of nano-SiOx/LZM/TP-CS composite coatings was prepared by the tape-casting method. Shewanella putrefaciens, one dominant spoilage bacteria of aquatic products, was used as the antibacterial object to study the antibacterial properties of the coatings. Scanning electron microscope (SEM) was used to observe the changes of the micro morphology of the bacteria before and after being treated by the composite preservative coatings. The changes of conductivities, OD260, Na+/K+-ATPase and AKP enzyme activities of the bacterial suspension treated by the composite preservative coatings for a certain time were determined. The results showed that all the coatings exhibited antibacterial properties. The bacteria deformed after being treated by the coatings, and the conductivity, OD260, Na+/K+-ATPase and AKP enzyme activities of the bacterial suspension were significantly higher than those of the untreated samples during the same period. After CS was modified by nano-SiOx, the antibacterial property of the coatings was slightly improved, the bacteria collapsed in many places, and the conductivity, OD260, Na+/K+-ATPase and AKP enzyme activities of bacterial suspension were significantly increased. After adding LZM or TP, the antibacterial properties of the coatings continued to improve, and the antibacterial properties of the in-situ synthesis of nano-SiOx/TP-CS composite coatings were better. When LZM and TP were added to CS coating modified by nano-SiOx at the same time, the cell wall was dissolved and seriously damaged. The conductivity and OD260 of the bacterial suspension treated at the same time were the highest, and even the Na+/K+-ATPase and AKP enzyme activities were inhibited. Combined with previous research results, SiOx/LZM/TP-CS composite preservative coatings have good preservative and antibacterial properties, which can provide theoretical guidance and technical support for the application of edible preservative coatings. |
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