高浩祥,梁恒宇,曾维才,石碧.植物多酚常见的定性定量分析方法[J].包装工程,2021,42(7):1-11.
GAO Hao-xiang,LIANG Heng-yu,ZENG Wei-cai,SHI Bi.Common Methods of Qualitative and Quantitative Analysis for Plant Polyphenols[J].Packaging Engineering,2021,42(7):1-11. |
| 植物多酚常见的定性定量分析方法 |
| Common Methods of Qualitative and Quantitative Analysis for Plant Polyphenols |
| 投稿时间：2020-09-19 |
| DOI：10.19554/j.cnki.1001-3563.2021.07.001 |
| 中文关键词: 植物多酚 定性分析 定量分析 |
| 英文关键词: plant polyphenols qualitative analysis quantitative analysis |
| 基金项目:国家自然科学基金(31801548);四川省科技计划(2021YFH0072);国家重点研发计划(2019YFE0103800) |
|
| 摘要点击次数: |
| 全文下载次数: |
| 中文摘要: |
| 目的 对植物多酚常见的定性定量分析方法进行综述，为其在食品包装和贮藏领域的资源化利用提供方法和实验支撑。方法 从实验原理、实际操作、适用范围和优缺点等方面对纸色谱法、薄层色谱法、显色法等常见的定性分析方法，以及高锰酸钾滴定法、酒石酸亚铁法、普鲁士蓝法、福林酚法、香草醛-盐酸法、正丁醇-盐酸法、近红外光谱法和原子吸收光谱法等常用的定量分析方法进行介绍和对比分析，对定量分析方法和测定标准品的选择进行进一步的介绍。结果 植物多酚常见的定性定量分析方法的原理和繁简程度各不相同，测试结果有较大差异，各有优缺点;在测定中，通常采取几种不同的分析方法对同一样品中的植物多酚含量进行定量测定，从而对样品中所含的不同结构和种类植物多酚的含量得到一个综合的表征与认识;在定量分析中，通常采用与样品中多酚类物质属于同一类的酚类化合物纯物质作为标准品进行测定。结论 研究者需要根据各自的研究目的和样品的特点，选择几种合适的方法构建一套合理的分析方案对植物多酚进行定性定量分析，从而全面地反映样品中植物多酚的类型和含量。 |
| 英文摘要: |
| In present study, the common qualitative and quantitative methods for plant polyphenols were reviewed, which could provide technology and experimental supports for its resource utilization in food packaging and storage. Based on the principle, procedure, application, advantages and disadvantages, the paper chromatography, thin layer chromatography, chromogenic method and other qualitative methods were reviewed. Meanwhile, the choice about the quantitative methods and standard compounds in determination were also introduced. The principle and complexity for all the qualitative and quantitative methods were different, and their testing results were also different, which both possessed advantages and disadvantages. Commonly, several methods should be used in quantitative determination for the same sample, so as to obtain the detailed information of plant polyphenols. In addition, some pure phenolic compounds as the plant polyphenols in sample were usually used as the standard. Therefore, researchers should design the analytical schemes by using several methods, which can reflect the type and content of plant polyphenols. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
渝公网安备 50010702501716号