江小剑.同位素内标-UPLC-MS/MS法检测易拉罐啤酒中双酚A含量[J].包装工程,2020,41(21):18-21.
JIANG Xiao-jian.Determination of Bisphenol A in Canned Beer by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry based on Isotope Internal Standard[J].Packaging Engineering,2020,41(21):18-21. |
| 同位素内标-UPLC-MS/MS法检测易拉罐啤酒中双酚A含量 |
| Determination of Bisphenol A in Canned Beer by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry based on Isotope Internal Standard |
| 投稿时间：2020-08-28 |
| DOI：10.19554/j.cnki.1001-3563.2020.21.003 |
| 中文关键词: 双酚A 超高效液相色谱/串联质谱法 同位素内标法 易拉罐啤酒 |
| 英文关键词: bisphenol A UPLC/MS-MS isotope internal standard canned beer |
| 基金项目:国家市场监督管理总局科技计划(2019MK081) |
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| 中文摘要: |
| 目的 为了提高金属易拉罐啤酒中双酚A(BPA)的检测能力，建立一种基于同位素内标的超高效液相色谱-串联质谱分析方法。方法 样品经超声处理后除去CO2，随后添加内标物BPA-13C12，用超高效液相色谱-串联质谱法进行检测分析，采用内标法定量。结果 经同位素内标标准曲线校正，方法的定量限为1.00 μg/L，线性方程为y=1.73x+0.206，线性相关系数为0.9992，相对标准偏差为4.68%～6.63%，加标回收率为91.0%～109%。使用该方法对厦门市市售6种易拉罐啤酒样品进行了BPA污染物迁移量检测，结果显示6种啤酒中均检出BPA，平均质量浓度为1.48 μg/L。结论 此方法具有前处理简便快捷、检测限高、灵敏度高、准确度好等特点，能够满足金属易拉罐啤酒中低含量BPA的检测要求。 |
| 英文摘要: |
| The work aims to establish a method based on isotope internal standard by ultra performance liquid chromatography-tandem mass spectrometry (UPLC/MS-MS) to enhance the determination ability of bisphenol A (BPA) in canned beer. The canned beer samples were mixed with BPA-13C12 after ultrasonic treatment, and then analyzed by UPLC/MS-MS and quantified by isotope-labelled internal standard. Quantified by the calibration curve of isotope-labelled internal standard, the detection limitation of the method was 1.00 μg/L. The linear equation was y=1.73x+0.206, the correlation coefficient was 0.9992, and the spiked recoveries of BPA was 91.0% to 109% with RSDs ranging from 4.68% to 6.63%. Then, the method was applied to determine the pollutant migration of BPA in 6 canned beers from Xiamen local market. The results showed that BPA was found in all samples with the average concentration of 1.48 μg/L. The method has the advantages of simple and fast pretreatment, low detection limitation, high sensitivity and good accuracy, which can satisfy the requirement of detecting low BPA concentration in canned beer. |
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