俞大海,毛佳甜,王一铭,徐丽广,马伟,刘丽强,匡华,胥传来.可食包装纸原料中玉米赤霉烯酮的快速检测研究[J].包装工程,2019,40(1):87-92.
YU Da-hai,MAO Jia-tian,WANG Yi-ming,XU Li-guang,MA Wei,LIU Li-qiang,KUANG Hua,XU Chuan-lai.Rapid Detection of Zearalenone in Edible Packaging Materials[J].Packaging Engineering,2019,40(1):87-92. |
| 可食包装纸原料中玉米赤霉烯酮的快速检测研究 |
| Rapid Detection of Zearalenone in Edible Packaging Materials |
| 投稿时间：2018-06-29 修订日期：2019-01-10 |
| DOI：10.19554/j.cnki.1001-3563.2019.01.013 |
| 中文关键词: 玉米赤霉烯酮 ELISA 胶体金 前处理 |
| 英文关键词: zearalenone ELISA colloidal gold pretreatment |
| 基金项目:国家自然科学基金面上项目（21673104）；国家自然科学基金重点研发项目（2017YFC1601102） |
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| 中文摘要: |
| 目的 针对可食包装纸原料中的玉米赤霉烯酮进行检测，采用以酶联免疫法为主，胶体金试纸条检测法为辅的免疫分析方法，为实时监控食品的包装、包装存储条件等提供有效技术手段。方法 实验主要选取可食包装纸原料玉米粉作为检测对象，为提高酶联免疫法检测结果的准确性，通过添加回收实验，以最高的回收率为判断标准对应得到最优的前处理条件；先通过标准曲线的封闭温度和温育时间的优化，再进行玉米赤霉烯酮的前处理方式（包括对提取液浓度、稀释液的pH值、稀释液中有机溶剂的浓度等3个方面进行优化，以添加回收结果作为判断依据）进行测试。同时，将优化后的实验结果组装到胶体金试纸条，并对掺杂玉米赤霉烯酮的玉米粉进行快速检测。结果 前处理最适条件，甲醇溶液的体积分数为50%，缓冲液pH值为8.0且含甲醇的体积分数为10%，在该条件下测得的回收率最高。在该优化条件下，组装的胶体金试纸条其检测灵敏度可达0.75 ng/mL。结论 该检测结果与添加标品所测实验结果基本一致，可以确定该胶体金试纸条是一种快速有效的检测方法。 |
| 英文摘要: |
| The work aims to detect zearalenone in edible packaging materials, and provide effective technical means for real-time monitoring of food packaging and packaging storage conditions, etc., mainly through enzyme-linked immunosorbent assays, supported by colloidal gold test strips. Corn flour (edible packaging material) was mainly selected as the experimental detection object. Through the addition recovery experiments, the maximum recovery rate was used to obtain the optimal pretreatment conditions, thereby improving the accuracy of the detection results of enzyme-linked immunosorbent assays. After optimizing the closed temperature and incubation time of the standard curve, the pretreatment method of zearalenone (including optimizing the concentration of the extractive solution, the pH value of the dilution and the concentration of organic solvent in the dilution, with the addition recovery result as the judgment basis) was tested. Meanwhile, the optimized experimental results were assembled into the colloidal gold test strips, and the zearalenone-containing corn flour was quickly detected. Through experiments, it was determined that the optimal conditions for the pretreatment were 50% (volume fraction) methanol solution, and buffer solution at pH 8.0 and with 10% (volume fraction) methanol. The recovery rate measured under this condition was the highest. In such optimal conditions, the detection limit of the assembled colloidal gold test strip could reach 0.75 ng/mL. The detection results are basically the same as the experimental results measured by addition level. It can be confirmed that the colloidal gold test strip is an effective rapid detection method. |
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